Ngwa ihe nchọpụta PCR SARS-CoV-2 na-adịgide adịgide (ojiji ụlọ)

Nkọwa dị mkpirikpi:

Iwebata ngwaahịa:

A na-eji ya nwalee ihe nlele a ga-anwale maka ebe pụrụ iche nke novel coronavirus nucleic acid (ORF1ab, Ngene).

Njirimara ngwaahịa:

• Ọ dị mfe: Ọ dị mfe ịrụ ọrụ, mụta na ịghọta, ọ nweghị ọzụzụ siri ike achọrọ.
•Isothermal: Chekwaa ọnụ ahịa akụrụngwa.
• Oke nkọwapụta:Dizi ezi nke ihe ruru 98%.
• Ngwa ngwa :Nchọpụta nwere ike mechaa n'ime 15 nkeji.
• Ụgbọ njem na nchekwa dị mma: ụgbọ njem na ebe nchekwa okpomọkụ, enweghị yinye oyi.

Nkọwapụta ngwaahịa:

1 ule/igbe16 ule/igbe

①swab②Swab nchekwa tube③Amplification mmeghachi omume tube④ metal bath


  • Aha ngwaahịa:Ngwa ihe nchọpụta PCR SARS-CoV-2 na-adịgide adịgide (ojiji ụlọ)
  • Ụdị:PCR okpomọkụ mgbe niile
  • Nkọwa nkwakọ ngwaahịa:1 ule/igbe, 16 ule/igbe
  • Nkọwa ngwaahịa

    Mkpado ngwaahịa

    Ụkpụrụ ule:
    Ngwa a na-achọpụta RNA nke SARS-CoV-2 site na iji usoro nkwalite isothermal.A na-eme ntụgharị ntụgharị na mplification nke RNA n'otu tube ahụ.A na-amata usoro nucleic acid nke SARS-CoV-2 nke ọma site na primers isii, na nkwekọ ọ bụla ma ọ bụ enweghị njikọ agaghị emecha nkwalite ahụ.A na-ebunye ya ihe niile reagents na enzymes achọrọ maka mmeghachi omume ahụ.Usoro dị mfe dị mkpa na nsonaazụ nwere ike nweta site na nleba anya na ọnụnọ ma ọ bụ na ọ bụghị nke fluorescence.

    Nkwadebe:

    Mepee akpa foil Aluminom wee wepụ tubes mmeghachi omume.Nlebara anya, a ga-eji tube mmeghachi omume n'ime awa 2 ozugbo emeghere akpa foil ya.

    Tinye ike.Ngwa ahụ na-amalite ikpo ọkụ (Ngosipụta kpo oku na-atụgharị uhie na ọkụ).Mgbe usoro kpo oku gasịrị, ihe ngosi ọkụ na-atụgharị akwụkwọ ndụ akwụkwọ ndụ na ụda.

    Nchịkọta ihe atụ:

    Tụgharịa isi onye ọrịa azụ ihe dị ka 70 Celsius, Ka isi onye ọrịa ahụ zuru ike nke ọma, ma jiri nwayọ tụgharịa swab ahụ na mgbidi nke enyí n'oghere imi onye ọrịa ruo n'ọnụ imi, wee jiri nwayọọ wepụ ya ka ọ na-ehichapụ ya.

    Nwale:
    ① Dịrị ihe nkiri mkpuchi mkpuchi aluminom nke Swab Preservation tube, ma tinye swab n'ime tube nchekwa swab.mgbe ị na-amanye tube, kpalie swab.
    ②Wepu swab mgbe ị na-amanye akụkụ nke tube iji wepụ mmiri mmiri na swab.
    ③Fanye micropipette ma tinye ya n'ime mmiri mmiri.Hapụ micropi-pette ka ọ dọpụta mmiri mmiri ruo mgbe mmiri mmiri na-asọba na capsule nke mbụ.Ekwela ka mmiri mmiri jupụta capsule nke mbụ.
    ④ Tinye ihe atụ mmiri n'ime tube mmeghachi omume, Mechie okpu ahụ, jiri nwayọọ gwakọta ngwakọta ahụ ruo mgbe agbazere kpamkpam.
    ⑤Mepee mkpuchi nke ịsa ahụ akọrọ.Tinye tubes mmeghachi omume mkpuchi n'ime bat akọrọ.Pịa bọtịnụ oge.Ihe ngosi kpo oku ndụ ndụ na-amalite ịgba ọkụ.Mgbe nkeji iri na ise gachara, emechara mmeghachi omume ahụ.Ihe ngosi kpo oku ndụ ndụ na-akwụsị iji beeps atọ na-egbuke egbuke.
    ⑥ Pịa bọtịnụ mgba ọkụ nke isi iyi ọkụ wee hụ nsonaazụ ule site na oghere nlele n'ihu bat akọrọ iji kpee nsonaazụ.
    Nkọwa nke nsonaazụ ule:

    N'ihi nti: ma ọ bụrụ na mmeghachi omume tube nwere doro anya green fluorescence excitation, N'ihi bụ positive.The ọrịa a na-enyo enyo na ọ bu ọrịa Sars-Cov-2.Kpọtụrụ dọkịta ozugbo ma ọ bụ ngalaba ahụike mpaghara wee soro ntuziaka mpaghara.
    N'ihi na-adịghị mma: ma ọ bụrụ na mmeghachi omume tube enweghị doro anya green fluorescence excitation, ya pụta bụ negative.Nọ n'ihu na-rube isi na niile na ọdabara iwu banyere kọntaktị na ndị ọzọ na-echebe usoro.E nwekwara ike ịbụ ọrịa mgbe anwale ọjọọ.
    Nsonaazụ na-ezighi ezi: ọ bụrụ na oge ntinye ahụ dị ogologo karịa 20 min, mmụba na-abụghị nke a kapịrị ọnụ nwere ike ime, na-eduga na ụgha ụgha.







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